Absorbance cuvette port. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Two high-energy xenon flash lamps: Reading capabilities. Two different agonists, acetylcholine and carbachol, were tested. 1 fmol luciferase per well. To prevent evaporation, the microplate was covered with a qPCR seal. Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. Microplate format. Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. Microarray scanners for one-fluor to multi-fluor [email protected] and Download Molecular Devices SPECTRAmax PLUS 384 operator's manual online. Filter. 00. [Carbachol] (nM) RFU Max. VIS absorbance detection. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as. Plate Formats: 6- to 384-well plates. 2. However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. The all new CloneSelect Imager FL features high contrast multichannel fluorescent and white light imaging that allows for accurate single cell detection and proof of monoclonality at day 0. 3% in white 96- and 384-well microplates Time. 3% in white 96- and 384-well microplates < 3 x 10-5 (white plates) < 3 x. Extended linear dynamic range. Includes PC and software. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. SpectraMax ID5 Plate Reader (Molecular Devices). Embla washers are durable and easy to maintain. 375 pM 1 FP Fluorescence Polarization detection for fluorescein- or rhodamine-like labels Using specific LED and EX/EM filters for 6- to 1536-well plates 0200-7009POS 0200-7010POS Fluorescein FP EX 485 nm, EM 535P and 535S nm Rhodamine FP EX 535 nm, EM 595P and 595S nm 96–1. 96- and 384-well . Three low volume 24-well bottom slides. Time-resolved phosphorescence. The opaque walls prevent stray light from being detected from neighbouring. If you have an xml plate object, then. xABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. Figure 2-23: Bottle holder To install the bottle holder: 1. The price of a microplate reader depends on the technical equipment and the number of measurement technologies that the device can measure. 20 attomol ATP per well. Rating: 3. is a welcome addition to any lab that is routinely measuring concentration or absorbance in 96 and/or 384 well plates. For more sample throughput, the system canThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. A Costar 24-well tissue culture plate with col1 = blanks and doubling cell5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12Reader Documentation Version No. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. A control well was set up to verify. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader Multimode Readers Comparison Chart. Figure 1. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. (1536-well plate) 2 µL (quartz cuvette), 4 µL (disposable cuvette). These models can no longer be repaired. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. ability to use as little as 2˜µL samples with 24- or 64-well plates. 384-Well Microarray/High Sample Recovery Plate. T = I/I 0 and %T = 100 (T). 1. and 25 μL of each concentration was added in triplicate to a 384-well solid white plate. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. 2. 6% for the on-board optics (monochromators). The SpectraMax 190 reader can be integrated with Molecular . x. Another consideration, says Amouretti, is plate format. The fluorescent intensity of each well was measured by using a fluorescence plate reader (SpectraMax Paradigm, Molecular Devices, Sunnyvale, CA, USA) at 588-nm excitation and 633-nm emission. Microplates for applications that require high sample recovery rates. Typically, the full spectrum of signal from. Multichannel processes: Costar 96-well (3300) and Greiner 384-well (781182/781185) microplates are validated for multichannel processes. Industry standard disposable (choose from recommended list). Note: These settings are based on necessary cookies. Wash heads are made of Polyethylene Terephthlate (PET) with stainless steel probes to prevent corrosion. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. 384-Well SBS Microplate, clear, square well, flat bottom, sterile, with cover, working volume 90 µl, packaged in sleeves of 10. FlexStation® 3 Multi-Mode MicroplateAlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. The washer has a small footprint and is vacuum and pressure free, The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. in every plate. Documentation:Molecular Devices microplate readers. Kinetic readings were started immediately with a Molecular Devices FlexStation Plate Reader. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Round well design with a solid black flat bottom2. F3/F5 Multi-Mode Microplate ReadersBoth reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. The plate reader optics are tunable for. Contact Us Phone: +1-800-635-5577 Web: Email: info@moldev. 100 fmol 384-well (75 µL) 0. x. 01% BSA starting at a concentration of 1 x 10 6 fg/well was prepared, and 25 μL of each concentration was added in triplicate to a 384-well solid white plate. Add the new plate. Plate chamber design of the SpectraMax category 1 microplate readers. ,LTD (CAT#: STEM-S. Total volume (except deep. After reading the ELISA plate on the EMax® Plus Microplate Reader, the R2 value was 0. Learn more about SpectraMax Plus 384 Microplate Reader >>Rating: 5. #ID5) HTRF Detection System (Molecular Devices cat. An HTRF assay measures the fluorescence energy transfer between molecules in close proximity. Page 1 of 8 . Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. Plates were read in the SpectraMax M5 microplate reader at 485ex/515em with bottom read. #3635) Transferpette micropipette (Drummond Scientific cat. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. Outer wells are aligned for proper viewing with FLIPR. x. x. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Microplate readers are commonly used in life science laboratories. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. M5. SpectraMax M Series Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. To address this concern, Molecular Devices offers the FlexStation® 3 Multi-Mode Microplate Reader. Reconstitution of reagents. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. com. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Loading Buffer was also added to a set of cell-free wells for background controls. x. The analyzed results are. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Two different agonists, acetylcholine and carbachol, were tested. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. 340 PC384. For Research Use Only. Find regional support. Typical 4 min/ 384 2 min/384 8 min/1536 Throughput plate plate plate Fully enabled protocols = reduced setup time. This can increase throughput and efficiency, thus saving time and space in the lab. (or plate reader). The estimated lower limit of detection (LLD) in both 96- and 384-well plates is < 2 fg/well for firefly luciferase in the LMax II 384 and Analyst GT and 2–3 fg/ well in the SpectraMax M5. For a higher . Catalog number: BC1384W. Made from polypropylene with conical well profiles to maximize recovery and allow working with sample volumes as low as 5 µL. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. It. plated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. B. plates < 100 amol (384-well) 1 0200-7019. #2704174, 2705402, 2705412, 2704180; Drummond Digital Microdispenser cat. This chapter is an introductory overview of the most commonly used assay methods to estimate the number of viable cells in multi-well plates. M5. use as little as 2 µL samples with 24- or 64-well plates. An IL-22 standard curve was prepared and a sandwich ELISA performed using the MultiWash+ plate washer in strip mode to wash the wells. It is recommended that you then centrifuge the plates at 1000 rpm for up to 4 minutes (brake off). Our microplate. The estimated lower limit of detection (LLD) in both 96- and 384-well plates is < 2 fg/well for firefly luciferase in the LMax II 384 and Analyst GT and 2–3 fg/ well in the SpectraMax M5. $402. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr: $32/hr. This eliminates the need for standard curves, and Flexibility to read a variety of plate formats. Molecular Devices: n/a:. Unmatched throughput FLIPR Membrane Potential Assay Kits allow the elimination of wash steps and much shorter read times on the instrument. the iD5 reader’s filter system. Download PDF. System setup and calibration are controlled from within SoftMax Pro Software. Molecular Devices 大中华区 Email: info. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. ©2022. (Component A, C) is sufficient for 1 plate (96-, 384-, 1536-well). FLIPR Calcium 4 Assay Kit (Molecular Devices, San Jose, CA, USA). explore more. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Contact Us Phone: +1-800-635-5577 Web: Email: [email protected] nM Z Factor at EC. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Published Dec 16, 2020 | Updated Dec 16, 2020. SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. The color touch screen provides a visual user interface, making programming fast and intuitive. ) 0. 384-WELL 10MM-HEIGHT HIGH SAMPLE RECOVERY MICROPLATES WI/WO LID. Outer wells aligned for proper viewing with FLIPR. Plate reader instructions Computer. Validated by Molecular Devices for the FLIPR reader. 635. 20 plates/hour (2-minute read time) 96-well 1,920 data points/hour 384-well 7,680 data points/hour 1536-well 30,720 data points/hour Bar code manual mode Keyboard entry Bar code remote mode Short or long side Temperature control Ambient +5°C to 40°C General specifications Dimensions (in. White plates reflect the light, enabling even low levels of Luminescence to be detected. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate: 0517 Lit. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Working in microplates allows for smaller assays that use less sample and reagent and increases throughput. Learn how the PathCheck feature can be applied to protein quantitation experiments using Molecular Devices absorbance-based microplate readers. These advertising cookies will no longer be set for this browser and device. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Unparalleled performance on a personalized platform. ©2017 Molecular Devices, LLC . X. 1 OD at 230 nm. 384-WELL SBS STERILE MICROPLATE. The. Suitable for use in BMG lab tech, Molecular Devices, Promega Glomax and other plate readers; Suitable for adhesive and heat sealing: our Moisture Barrier Seal 384. EPOCH2 Plate Reader/Spectrophotometer. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. 96-deep-round-well-plate, 384-deep-square-well-plate, 24-deep-well-plates. x. Turn the. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the. Four wash/rinse bottles are included and configurable for both 96- and 384. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. Summary of Contents for Molecular Devices SpectraMax M2. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. * Each reagent vial (Component A) is sufficient for 1 plate (96-, 384-, 1536-well). , April 14, 2022 – Molecular Devices, LLC. 057, depending on lot number. The Embla 96/384 Well Washer accepts 96-and 384-well format microplates and is ideal. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. We would like to show you a description here but the site won’t allow us. Pfa1 and Pfa1 Gpx4-KO cells stably overexpressing murine FSP1 were seeded on separate 384-well plates (500 cells per well), and screened with a library of small-molecule inhibitor compounds, as. This kit is designed for customers who. Recertification service. The assay methods covered include. 0 . 2. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. tools to expand the boundaries of their research. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the. Select Yes at the warning. Microplate Readers Molecular Devices Spectramax M3 Multi Mode Microplate Reader. Ensure data accuracy of your reader. minutes and begin reading microplates with seven mouse clicks. 0 OD for our latest microplate readers, SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. If no reference reading is taken, the instrument uses the I 0 values stored in the NVRAM (non-volatile memory) of the. Experience the identifiably different. 0 mP 384–1. FLIPR Calcium 4 Assay Kit. Gemini EM Reader scans. Next generation SpectraMax® absorbance microplate readers. 7°C and the bottom to 36. the use of a reader calibrated with primary NIST and NMI standards. 9. We would like to show you a description here but the site won’t allow us. A ten-fold serial dilution of purified luciferase in PBS with 0. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. +1 800. External Dimensions without lid (mm). Dimensions: 18. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. Microplate format. Temperature control: 4°C above ambient to 45°C. Bioz Stars score: 86/100, based on 9 PubMed citations. Molecular Devices deliver high throughput while showing good correlation with manual patch clamping data. Important things to consider when purchasing a multimode microplate reader. 3. 384 Well Optically Clear Tissue Culture Plate: Plate length: 127. Ensure data accuracy of your reader. The eight-channel system delivers both high precision and speed when reading 96- and 384-well microplates for endpoint measurements, kinetic assays, and spectral scans. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. Plate Formats: 6- to 384-well plates, NanoQuant Plate (Sold Separately) Detection Modes: Luminescence, Fluorescence, Absorbance. 100 µL (96-well assay) or 25 µL (384-well assay) of CellTiter-Glo reagent was added to each well. EMax. #4TI-0655) Microplates. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. Instrument settings are listed in Table 1. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. Pick the best speed, sensitivity, and price for your needs. x. Supersedes 1. For more sample throughput, the system canSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. SpectraMax® ABS Plus microplate reader can run both cuvette-based and microplate reader applications on the same instrument. #2704174, 2705402, 2705412, 2704180; Drummond Digital Microdispenser cat. Click SoftMax Pro Software User Guide. 384- or 96-well read plate containing cells loaded. 384 well polystyrene microplates have plate dimensions of 127. These lab workhorses are used in drug discovery, research, bioassay validation, and. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Key benefits • Assess cell confluence objectively and quantitatively. $542. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Note: if all wells of a 384-well plate are to be read, the kinetic interval may need to be. It comes standard with 4 wash/rinse bottles and 1 waste bottle for maximum washing flexibility. That explains the lower values on the LmaxII384 plate reader. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). Microplates, cuvettes (via adapter) Detectors. 384-Well Microarray/High Sample Recovery Plate. Evaporation protection (humidity cassette) Te-Cool™ (active temperature regulation 18-42 °C) Integrated lid handling. X. Tb. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. plates < 100 amol (384-well) 1 0200-7018. A rinse program is included for quick and easy daily. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. Spectranet customer portalThe microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. Our washers provide speed and configurability options—from single-tube dispensing to washing full 96- and 384-well plates. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. Service Contracts, Preventative Maintenance and. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. We would like to show you a description here but the site won’t allow us. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Access this by going to. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell concentration left to right starting at Col. QC tools and IQOQ services. Instructions for adding new plates: CSI/CP2: Add plate. x. 1 fmol luciferase per well. Not for use in diagnostic procedures. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. San Jose, CA 95134. 2. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Prepare human iPSC-derived neuronal progenitor cells (NPCs) according to Shi et al. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 8mm distance between well bottoms to plate bottom 50 µL total well volume, with recommended working volume of 5-40 µL High base plate design is compatible with automation and for bar coding Validated by Molecular Devices for the FLIPR reader. ©2013 Molecular Devices, LLC • 6/13 • Printed in USA • PN: 0120-1523. changes in OD 600 were measured by periodically transferring 100-µL aliquots of each bacterial culture to a single well of a 96-well microplate and measuring absorbance at 600 nm using the SpectraMax iD3 reader and SoftMax Pro Software. Analyst, M5, Flex 3, and SpectraMax L readers work differently. 1. UV absorbance detection. 6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. Published Dec 16, 2020 | Updated Dec 16, 2020 Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader What is the minimum amount of DNA needed. Tunable wavelength selection / monochromator. Prepare human iPSC-derived neuronal progenitor cells (NPCs) according to Shi et al. Microplate Spectrophotometer. SpectraMax Mini Multi-Mode Microplate Reader (Molecular Devices P/N SMAX MINI AF), with fluorescence filter cube FL-535 (Molecular Devices P/N 5089097). 5577. Versa Max. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. Thermo Scientific Pierce Streptavidin Coated Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays. 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. The purposes of the study were: 1) to determine the optimal wavelength settings for three cell lines. #PLUS 384) Methods. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. After incubation, the assay plate is transferred to the seal-peeler to remove the seal from the assay plate and transferred to washing. CO 2 & O 2 control. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. All measurements were performed in six replicates and cell toxicity was calculated as the percentage of the OD value of untreated or treated. Polypropylene deep well plates: Elevated well walls for easier sealing Round. 0 (or higher) Software; Methods Data acquisition using the Workflow Editor. 76 L x 85. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. Microplate readers from Tecan; cutting edge bench-top microplate readers lead the industry in both versatility and performance. Test up to 3. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. 00. x. 190. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell concentration left to right starting at Col. REF - For the SpectraMax Plus 384, press to do a Cuvette Reference correction method read of buffer, water, or air taken in the cuvette that is used as I to calculate Absorbance or % Transmittance with the PathCheck Pathlength Measurement Technology. Selects either 96-well or 384-well plate for a stand-alone read. 0 mP 96-well. . Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. A snapshot of the Settings screen is shown in Figure 2. 4 decades . 96- and 384-well plates; Wavelength(s): 635 nm (classification), 532 nm (reporter) Citations:. Gemini EM Reader scans. 9. Method 1. Use standard plate unless you see your type listed. 96 deep well plate: packed in sleeves of 6 , 24 per case. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Important things to consider when purchasing a multimode microplate reader. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent. Turn on Plate Reader and wait for it to complete its start-up routine. M5. In addition, SoftMax Pro SoftwareBelow is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. x. readers are the industry’s most cited instruments and have empowered life science researchers to advance protein and cell biology, breaking the barriers to novel, landmark discoveries. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. • Easy and gentle immobilization of biotin. The Embla 96/384 Well Washer accepts 96-and 384-well format microplates and is ideal. 11 10 100 1000 0 20 40 60 80 100 120 Calcium Mobilization in WT3M1 CHO Cells EC 50 = 13. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. Kinetic assays were read every 20-28 seconds for one hour,measurements in compatible Molecular Devices® microplate readers.